Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum (RS) is one of the most serious tobacco
diseases worldwide and no effective control measures are available to date. This study investigated the
potential of Trichoderma harzianum SQR-T037 amended bioorganic fertilizer (BOF) and the arbuscular
mycorrhizal fungi (AMF) Glomus mosseae 171 (Gm) on the control of TBW and promotion of plant growth
in pot experiments. The results showed that the disease incidence in plants treated with integrated application
of G. mosseae 171 and T. harzianum SQR-T037 amended bioorganic fertilizer (Gm + BOF) was the
lowest, with a control efficacy of 68.2%, which is greater than that of BOF or Gm alone (26.8% and
14.7%, respectively). The application of BOF or Gm alone significantly reduced the abundance of RS in rhizosphere
soil, but the integrated treatment (Gm + BOF) showed the strongest inhibitory effect (with a
21.3% increase in inhibition). The root colonization of G. mosseae 171 in samples treated with Gm
+ BOF was higher than that in samples with solely Gm treatment, indicating that the BOF significantly
promoted G. mosseae mycorrhizal colonization. The results showed that the G. mosseae also had a positive
effect on SQR-T037 rhizospheric colonization. Denaturing gradient gel electrophoresis (DGGE) results
showed that application of BOF and Gm alone or in combination changed the diversity of the rhizospheric
microbial community. The integrated application of Gm + BOF to tobacco plants significantly increases
the activity of polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), and peroxidase (POD),
enzymes associated to systemic resistance. Additionally, the integrated application of Gm with BOF
increased the tobacco plant height, shoot dry weight, and root dry weight. In conclusion, a synergistic
biological approach integrating application of Gm and BOF for TBW protection seems promising.