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Each treatment (two earthworm speciesfour seed species)was replicated ten times. Thereafter, earthworms were removedand their gut voided in a Petri dish with moistfilter paper (48 h,15 C, darkness). Casts were broken down. Seeds that transitedthrough the earthworm gut and that were found in casts werecounted as ingested and excreted. Seeds that were not found in castswere considered as non-ingested. For each earthworm individual,the difference between the number of introduced seeds (20) and thenumbers of excreted seeds and of non-ingested seeds was taken asthe number of totally digested seeds. We did not make thedistinction between seeds that were crushed in the gizzard andchemically digested seeds. All seeds that were not recovered willbe referred to as digested hereafter. Ingested seeds are eitherexcreted or digested seeds. The level of digestion of excreted seedsafter their passage through earthworm gut was not evaluated. Thesorted seeds were placed in a controlled chamber (24 C, light/dark,18/6 h) and their germination was monitored for 14 days.
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