4. Conclusions
This study is the first to apply PCR-RFLP analysis to QIAxcel
automated capillary electrophoresis to identify species of Cyprinidae
fish and Cyprinidae-related processed products. In this study,
the PCR-RFLP profiles of 10 fresh Cyprinidae fish were established,
and the primer set of CypbL/CypbH was used to amplify a 331-bp
fragment from heavily processed products (121 C for 30 min and
160 C for 9 min). The coupling of PCR-RFLP with CE was shown to
be a good tool for determining the correct species of fish present in
processed fish products in Taiwan. This method is a safe, fast, and
efficient approach that uses fewer reagents and smaller samples for
the routine identification of species