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4.2. Agar dilution method The agar dilution method involves the incorporation of varying desired concentrations of the antimicrobial agent into an agar medium (molten agar medium),habitually using serial two-fold dilutions, followed by the inoculation of a defined microbial inoculum on to the agar plate surface.The MIC endpoint is recorded as the lowest concentration of antimicrobial agent that completely inhibits growth under suitable incubation conditions (Table1). This technique is suitable for both antibacterial and antifungal susceptibility testing.If multiple isolates are being tested against a single compound,or if the compound (or extract)tested masks the detection of microbial growth in the liquid medium with its coloring, agar dilution method is often preferred to broth dilution for the MIC determination. Nowadays,commercially produced inoculum replicators are available and can transfer between 32 and 60 different bacterial inoculato each agar plate.Agar dilution is often recommended as a standardized method for fastidious organisms [79] such as an aerobes and Helicobacter species. It has been also used for antifungal agent-drugs combinations against Candida sp., Aspergillus, Fusarium and dermatophytes [80–83]. This method presents agoodc or relation with E test mostly for antibacterial testing against both Gram-positive and Gram-negative bacteria. More over,category comparisons of agar dilution, disk-diffusion and broth micro dilution methods give excellent results [25].
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