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One kg of dry, mature, whole soybeans was soaked in 3 Ldistilled water at 25 8C for 24 h. The soak water was decantedand the beans washed and ground in 6 L boiling distilled waterin a blender (Waring Division, Dynamics Corporation,New Hartford, Conn., U.S.A.). The resulting suspension wasfiltered through 3 layers of cheesecloth, autoclaved for 15min at 121 8C and stored at 4 8C until required
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