All the experiments were performedin a 7.5 L capacity fermenter
with 4.5 L working volume (Labfors, HT Bottmingen, Switzerland).
APS having 25 g/L suspended solids (SS) concentration was used as a
substrate for CA bioproduction. APS was sterilized at 121 ± 1
◦
C for
30 min. After sterilization, the substrate was supplemented with
inducers [3% (v/v) EtOH and MeOH] in different treatment. The
medium was inoculated with 10% (v/v) inoculum concentration.
Temperature and pH of the fermentation medium was controlled
at 30 ± 1
◦
C and 3.5, respectively. To maintain dissolved oxygen con-centration above 20% saturation (critical oxygen concentration),
the medium was agitated at a speed of 300 rpm, and the air flow
rate was automatically controlled using a computer controlled sys-tem. Samples were withdrawn from the fermenter at 24 h intervals
for the CA and biomass estimation. All the experiments were per-formed in triplicates and the values given were average along with
standard deviation