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We used the anecic L. terrestris L. and the endogeic A. chlorotica(Sav.) and Aporrectodea rosea (Sav.) that are commonly found ingrassland ecosystems of North–West France (Decaëns et al., 2008).Anecic earthworms feed on plant litter and contribute to theincorporation of soil organic matter into deeper soil layers via their vertical movement and endogeic species mostly feed on soilorganic matter (Lee, 1985). A. chlorotica individuals (AC;0.320.08 g, average fresh weight) were hand-sampled in April2011 in grasslands outside the university campus of Mont-Saint-Aignan. A. rosea individuals (AR; 0.230.04 g) were hand-sampledin alluvial deposits near the Seine River and L. terrestris individuals(LT; 5.230.73 g) were purchased in afishing bait store. Aftervoiding their guts for 24h on moistfilter paper in Petri dishes, threeadult individuals from a single species were added to eachmicrocosm leading to a total of twelve treatments: 3 soil types(casts from 3 earthworm species + no-worm control casts). Eachtreatment was replicatedfive times and all 60 microcosms werekept in darkness at 17 C for the length of the experiment
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