A standard β-galactosidase expressi

A standard β-galactosidase expression assay was performed to estimate
the effect of chitosan on the efficiency of protein translation
(Alamgir et al., 2008). The assay employs the vector p416 (Mumberg
et al., 1994) which contains the yeast GAL1 promoter controlling
lacZ (β-galactosidase) expression; transcription of lacZ, and thus
β-galactosidase biosynthesis, is repressed in the presence of glucose
and induced with galactose. The vector p416 was transformed into
the yeast strain W303. The transformed yeast cells were grown in
synthetic medium lacking uracil (SC-URA with glucose) and incubated
at 30 °C for 24 h, before resuspending in SC-URA with galactose.
The cultures were adjusted to an OD600nm ≈ 0.7 and incubated for 8 h
at 30 °C with or without a range of sub-inhibitory concentrations
of chitosan (0.35–1.25 mg/ml). A known protein synthesis inhibitor,

Results (Indonesian) 2:

Sebuah uji ekspresi β-galaktosidase standar dilakukan untuk memperkirakan
efek dari chitosan pada efisiensi translasi protein
(Alamgir et al., 2008). Uji mempekerjakan p416 vektor (Mumberg
et al., 1994) yang berisi promotor ragi GAL1 mengendalikan
lacZ (β-galaktosidase) ekspresi; transkripsi lacZ, dan dengan demikian
biosintesis β-galaktosidase, ditekan dengan adanya glukosa
dan diinduksi dengan galaktosa. Vektor p416 diubah menjadi
satu W303 ragi ketegangan. Sel-sel ragi berubah ditumbuhkan dalam
media sintetis kurang urasil (SC-URA dengan glukosa) dan diinkubasi
pada suhu 30 ° C selama 24 jam, sebelum resuspending di SC-URA dengan galaktosa.
Kultur disesuaikan ke OD600nm ≈ 0,7 dan diinkubasi selama 8 jam
pada suhu 30 ° C dengan atau tanpa kisaran konsentrasi sub-hambat
kitosan (0,35-1,25 mg / ml). Sebuah dikenal sintesis protein inhibitor,