Solvent evaporation is a simple method and the most commonly used to fabricate PHBHHx films (34).
In detail, a PHBHHx solution is prepared by dissolving purified PHBHHx in chloroform, which is then evenly distributed into glass dishes.
The chloroform is allowed to evaporate at room temperature, resulting in the formation of PHBHHx films on the Petri dishes.
Vacuum drying is generally applied to eliminate the residual solvent, because of its cytotoxicity