Detection for carbohydrates and gly

Detection for carbohydrates and glycosides Molisch’s test 10 g of alpha naphthol was dissolved in 100 ml of 95% alcohol. Extract was treated with this solution and 0.2 ml of conc. sulphuric acid was slowly added through the sides of the test tube, purple or violet color appeared at the junction.
Benedicts test
The test solution was treated with few drops of Benedict’s reagent (alkaline solution containing cupric citrate complex) and upon boiling on water bath, reddish brown precipitate formed if reducing sugars were present.Fehling’s Test
6.932 g of copper sulphate was dissolved in distilled water and make volume upto 100 ml (solution A). 34.6 g of potassium sodium tartarate and 10
g of sodium hydroxide was dissolved in distilled water and make volume up to 100 ml (solution B). Two solution was mixed in equal volume prior to use and few drop of sample was added and boiled, a brick red precipitate of cuprous oxide was formed, if reducing sugars were present.

Test for sterols and triterpenoids
Salkowski test Extract was treated with few drops of conc. Sulfuric acid shake well and allowed to stand for some time, red color appear at the lower layer indicated the presence of steroids and formation of yellow colored lower layer indicated the presence of triterpenoids.Sulphur powder test
Small amount of sulphur powder was added to the test solution, it sinks at the bottom.

Test for proteins and amino acids
Million test 1g of mercury was dissolved in 9 ml of fuming nitric acid,keeping the mixture well cooled during the reaction. When the reaction was completed, equal volume of distilled water was added. 2ml Million reagent was added to the extract gave white precipitate which turns red upon gentle heating.

Ninhydrin test
1g of ninhydrin (indane1,2,3 trione hydrate) was dissolved
in n-butanol and make the volume to 100ml. Extract treated
with this solution gave violet color on boiling.

Biuret test
To 3ml test solution 4% w/v NaOH
and few drops of 1% w/v copper sulphate solution were added. A blue color was observed.

Test for sponins
Foam test 1ml of extract was diluted with distilled water to 20
ml and shake in a graduated cylinder for 15 minutes. A one
centimeter layer of foam indicated the presence of sponins.

Test for tannins and phenolic compounds
Ferric chloride test Extract was treated with ferric chloride solution, blue color was appeared if hydrolysable tannin was present and green
color was appeared if condensed tannins was present

Results (Indonesian) 2:

Deteksi untuk karbohidrat dan glikosida Molisch test 10 g alpha naftol dilarutkan dalam 100 ml alkohol 95%. Ekstrak diperlakukan dengan solusi ini dan 0,2 ml conc. asam sulfat perlahan-lahan ditambahkan melalui sisi tabung tes, ungu atau violet warna muncul di persimpangan.
Benedicts menguji
Solusi uji diobati dengan beberapa tetes larutan benedict (larutan alkali yang mengandung kompleks sitrat tembaga) dan setelah mendidih pada air mandi, kemerahan endapan coklat terbentuk jika gula pereduksi yang present.Fehling Uji
6,932 g tembaga sulfat dilarutkan dalam air suling dan membuat volume yang upto 100 ml (larutan A). 34,6 g kalium natrium tartrate dan 10
g natrium hidroksida dilarutkan dalam air suling dan membuat volume ke atas untuk 100 ml (larutan B). Dua solusi dicampur dalam volume yang sama sebelum digunakan dan beberapa tetes sampel ditambahkan dan direbus, batu bata endapan merah oksida tembaga dibentuk, jika mengurangi gula hadir. Uji sterol dan triterpenoid Salkowski uji Ekstrak diperlakukan dengan beberapa tetes pekat. Asam sulfat kocok dan didiamkan selama beberapa waktu, warna merah muncul di lapisan bawah menunjukkan adanya steroid dan pembentukan kuning berwarna lapisan bawah menunjukkan adanya triterpenoids.Sulphur uji bubuk jumlah kecil bubuk belerang ditambahkan untuk menguji solusi, tenggelam di bagian bawah. uji protein dan asam amino Juta tes 1g merkuri dilarutkan dalam 9 ml asam nitrat, menjaga campuran baik didinginkan selama reaksi. Ketika reaksi selesai, volume yang sama dari air suling ditambahkan. 2ml Juta reagen ditambahkan ke ekstrak memberi endapan putih yang berubah merah pada pemanasan lembut. Uji Ninhydrin 1g dari ninhidrin (indane1,2,3 trione hydrate) dilarutkan dalam n-butanol dan membuat volume sampai 100 ml. Ekstrak diperlakukan dengan solusi ini memberi warna violet pada mendidih. Uji Biuret Untuk larutan uji 3ml 4% w / v NaOH dan beberapa tetes 1% b / v larutan tembaga sulfat ditambahkan. Sebuah warna biru diamati. Uji sponins Foam tes 1ml ekstrak diencerkan dengan air suling untuk 20 ml dan kocok dalam silinder lulus selama 15 menit. Sebuah satu lapisan sentimeter busa menunjukkan adanya sponins. Uji tanin dan senyawa fenolik Ferri uji klorida Ekstrak diperlakukan dengan larutan klorida, warna biru muncul jika tanin terhidrolisa hadir dan hijau warna yang muncul jika tannin hadir