Biotechnology can complement conventional breeding and expedite the mango improvement programmes. Studies involving in
vitro culture and selection, micropropagation, embryo rescue, genetic transformation, marker-assisted characterization and DNA
fingerprinting, etc. are underway at different centers worldwide. In vitro culture and somatic embryogenesis of several different
genotypes have been achieved. The nucellus excised from immature fruitlets is the appropriate explant for induction of embryogenic
cultures. High frequency somatic embryogenesis has been achieved in some genotypes; however, some abnormalities
can occur during somatic embryo germination. Embryo rescue from young and dropped fruitlets can improve the hybridization
success in a limited flowering season. Protocols for protoplast culture and regeneration have also been developed. In vitro
selections for antibiotic tolerance and fungal toxin resistance have been very promising for germplasm screening. Genetic
transformation using Agrobacterium tumefaciens has been reported. Genes that are involved with fruit ripening have been cloned
and there have been attempts to deliver these genes into plants. DNA fingerprinting and studies on genetic diversity of mango
cultivars and Mangifera species are also being conducted at several research stations. The purpose of this review is to focus upon
contemporary information on biotechnological advances made in mango. It also describes some ways of overcoming the problems
encountered during in vitro propagation of mang