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A number of specialized xylanase assays have been developed that make useof dyed or derivatized substrates. Carboxymethyl xylan will differentiatebetween xylanases that react with the xylan backbone and those that removeside chains (Khowala et al. 1988). Assays based on dyed substrates are oftenmore sensitive, but the results are also more difficult to interpret from a kineticperspective (Schmidt and Kebernik 1988). Dyed substrates are particularlyuseful in developing zymograms from electrophoresis gels (Biely et al. 1985a,1988). As with all unnatural or substituted substrates, care must be taken toassure that the assay reflects the activity sought. Methods used to assayxylanase activities in various laboratories around the world were summarizedby Ghose and Bisaria (1987).
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