With few exceptions, most part of information concerning to candidal physiology, genetics, and virulence has been almost exclusively been obtained by experimentation under aerobic conditions and positive Eh values. As C. albicans has been isolated from anoxic sites, it is mandatory that more attention be dispensed to the environmental conditioning growth. For this, appropriate protocols must be proposed. As far as we know, Dumitru et al. (2004) were the first group to attempt to this and pointed out the necessity to adequate conditions for candidal growth when dealing with imidazoles and polyenic drugs to be used in anaerobic infections. After this, Semprebom et al. (2009) proposed that standardized anoxic cultures may be employed in studies with antiseptics as chlorhexidine.