enzyme in the antioxidant system of

enzyme in the antioxidant system of body to scavenge ROS indirectly,
or inhibiting the expression and activity of oxidase. Therefore,
taking together of the results of in vitro and in vivo tests,
flavonoids rich extract of R. tomentosa berries could exhibit antioxidant
activity through direct scavenging effect on ROS as well as
promote the expression of antioxidant enzyme to scavenge ROS.
In all, flavonoids rich extract from R. tomentosa berries might be
used as natural antioxidants and alternatives to prepare anti-ageing
reagent.
3.4. Identification of total flavonoids extract
In this study, UPLC–TOF-MS/MS was used to determine the
chemical compositions in the flavonoids rich extract from R. tomentosa
berries. The extraction ion chromatograms and mass spectrum
for tentative flavonoids were shown in Figs. 2 and 3,
respectively. Table 2 contained their formula, retention times,
MW, MS and MS2 fragmentation ions. Six flavonoids exhibited
their quasi-molecular ions [M+H]+. Based on their typical fragmentation
patterns as previously reported (Bonaccorsi, Caristi,
Gargiulli, & Leuzzi, 2010; Ohkoshi et al., 2007; Stevens, Hart,
Elema, & Bolck, 1996), compound 1 (tR = 5.896 min), with a molecular
iron at m/z 287 (C15H10O6), was tentatively assigned to
kaempferol with a characteristic fragment ion at m/z 171
([MHC4H4O4]+); compound 2 (tR = 6.319 min), with [MH]+ at
m/z 627 (C27H30O17), was tentatively identified as quercetin-7,40-
diglucoside and two fragment ions at m/z 465 and 303 were corresponded
to quercetin-7-glucoside and quercetin, respectively;
compound 3 (tR = 6.648 min) had a molecular ion at m/z 321
(C27H30O17) and two fragment ions m/z 155 [MC8H6O4]+ and
137 [MC8H6O4H2O]+ tentatively indicated that compound 3
was dihydromyricetin; compound 4 (tR = 6.758 min), with M+ at
m/z 433 (C21H20O10), was tentatively identified as vitexin and fragment
ions at m/z 415 and 295 were corresponded to [MH2O]+ and
[MH2OC4H8O4]+, respectively; compound 5 (tR = 6.900 min),
produced a [MH]+ at m/z 319 (C15H10O8) which was fragmented
into one major fragment at m/z 153 [MC8H6O4]+, was tentatively
identified as myricetin; compound 6 (tR = 7.474 min), exhibited a

Results (Indonesian) 2:

enzim dalam sistem antioksidan tubuh untuk mengais ROS secara tidak langsung,
atau menghambat ekspresi dan aktivitas oksidase. Oleh karena itu,
mengambil bersama dari hasil in vitro dan in vivo,
flavonoid ekstrak kaya R. tomentosa berry bisa menunjukkan antioksidan
aktivitas melalui efek scavenging langsung pada ROS serta
mempromosikan ekspresi enzim antioksidan untuk mengais ROS.
Dalam semua, flavonoid ekstrak kaya dari R. tomentosa berries mungkin
digunakan sebagai antioksidan alami dan alternatif untuk mempersiapkan anti-penuaan
reagen.
3.4. Identifikasi total flavonoid ekstrak
Dalam studi ini, UPLC-TOF-MS / MS digunakan untuk menentukan
komposisi kimia dalam flavonoid ekstrak kaya dari R. tomentosa
buah. Kromatogram ion ekstraksi dan massa spektrum
untuk flavonoid tentatif ditunjukkan pada Gambar. 2 dan 3,
masing-masing. Tabel 2 berisi rumus mereka, waktu retensi,
MW, MS dan ion fragmentasi MS2. Enam flavonoid dipamerkan
kuasi-molekul ion mereka [M + H] +. Berdasarkan fragmentasi khas mereka
pola seperti dilaporkan sebelumnya (Bonaccorsi, Caristi,
Gargiulli, & Leuzzi, 2010; Ohkoshi et al, 2007;. Stevens, Hart,
Elema, & bolck, 1996), senyawa 1 (tR = 5,896 min), dengan molekul
besi pada m / z 287 (C15H10O6), secara tentatif ditugaskan untuk
kaempferol dengan ion fragmen karakteristik pada m / z 171
([M H C4H4O4??] +); Senyawa 2 (tR = 6,319 min), dengan [M? H] + pada
m / z 627 (C27H30O17), itu sementara diidentifikasi sebagai quercetin-7,40-
diglucoside dan dua fragmen ion pada m / z 465 dan 303 yang berhubungan
dengan quercetin-7-glukosida dan quercetin, masing-masing;
senyawa 3 (tR = 6.648 menit) memiliki ion molekul pada m / z 321
(C27H30O17) dan dua ion fragmen m / z 155 [M C8H6O4?] + dan
137 [M C8H6O4? ? H2O] + tentatif menunjukkan bahwa senyawa 3
adalah dihydromyricetin; Senyawa 4 (tR = 6,758 min), dengan M + di
m / z 433 (C21H20O10), itu sementara diidentifikasi sebagai vitexin dan fragmen
ion pada m / z 415 dan 295 yang berhubungan dengan [M? H2O] + dan
[M? H2O? C4H8O4] +, masing-masing; senyawa 5 (tR = 6.900 menit),
menghasilkan [M H?] + pada m / z 319 (C15H10O8) yang terfragmentasi
menjadi satu fragmen besar pada m / z 153 [M C8H6O4?] +, itu sementara
diidentifikasi sebagai myricetin; Senyawa 6 (tR = 7,474 min), dipamerkan