Laboratory scale purification for α-amylase includes various combinations of ion exchange, gel filtration, hydrophobicity interactions and reverse phase chromatography
Liquid–liquid extraction (LLE) consists in transferring one (or more) solute(s) contained in a feed solution to another immiscible liquid (solvent). The solvent that is enriched in solute(s) is called extract. The feed solution that is depleted in solute(s) is called raffinate.