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The most exacting method for evaluating hemicellulase activities is toseparate and characterize the products. This can be accomplished by paperchromatography (e.g. Biely et al. 1980) or by thin layer chromatography (e.g.Morosoli et al. 1986), but in order to perform quantitative analyses, separationmust be accomplished by HPLC. In the past, relatively few HPLC columnswere adequate for separating complex oligosaccharides.
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