The stems of Dendrobium officinale

The stems of Dendrobium officinale Kimura et Migo, named Tie-pi-shi-hu,
is one of the most endangered and precious species in China. Because of its various
pharmacodynamic effects, D. officinale is widely recognized as a high-quality health food
in China and other countries in south and south-east Asia. With the rising interest of
D. officinale, its products have a high price due to a limited supply. This high price has led
to the proliferation of adulterants in the market. To ensure the safe use of D. officinale, a
fast and convenient method combining normal and fluorescence microscopy was applied
in the present study to distinguish D. officinale from three commonly used adulterants
including Zi-pi-shi-hu (D. devonianum), Shui-cao-shi-hu (D. aphyllum), Guang-jie-shi-hu
(D. gratiosissimum). The result demonstrated that D. officinale could be identified by the
characteristic “two hat-shaped” vascular bundle sheath observed under the fluorescence
microscopy and the distribution of raphides under normal light microscopy. The other three
adulterants could be discriminated by the vascular bundle differences and the distribution of
raphides under normal light microscopy. This work indicated that combination of normal light
and fluorescence microscopy is a fast and efficient technique to scientifically distinguish
D. officinale from the commonly confused species.

Results (Chinese) 2:

铁皮石斛石斛，命名为茎铁丕市虎，
是最濒危的和珍贵的物种之一的中国。由于其不同的
药效学作用，铁皮石斛是目前公认的一种高品质的保健食品
在中国和其他国家在南部和东南部地区。随着利率上升
D. 铁皮石斛，其产品具有很高的性价比，由于限量供应。这种高价位，导致
在市场上伪品泛滥。为确保安全使用D的铁皮石斛，一个
快速，方便的方法相结合的正常和荧光显微镜应用于
本研究从三个常用的伪品鉴别铁皮石斛
包括子丕市虎（D. devonianum）水草市虎（D. aphyllum），广杰市虎
（D. gratiosissimum）。结果表明，铁皮石斛可以通过识别
特征“两帽形”维管束下的荧光观察鞘
显微镜和针晶在正常光镜的分布。其他三个
混淆品可以通过维管束差异和分布受到歧视
针晶在正常的光学显微镜。这项工作表明，正常光线的组合
和荧光显微镜是一种快速，高效的技术，科学的区分
D. 铁皮从经常混淆的品种。

Results (Chinese) 3:

铁皮石斛的茎，叫铁皮石湖，
是中国最濒危的和珍贵的物种之一。因为它的各种
药效学作用，铁皮石斛是公认的在南亚和东南亚在中国和其他国家的优质健康食品
。随着利率的上升
铁皮石斛，其产品有较高的价格，由于供应有限。这样高的价格
LED市场中掺假的增殖。为了保证铁皮石斛的安全使用，一
快捷方便结合正常和荧光显微镜在鉴别铁皮石斛从常用的三种伪品的
包括紫皮石湖目前的研究应用
方法（齿瓣石斛），水曹石湖（D. Aphyllum），广街石湖
（D. gratiosissimum）。结果表明，铁皮石斛可以由
特征”两个帽子状维管束鞘的荧光显微镜下观察
正常光镜下细胞分布的确定。其他三个伪品可以由维管束差异和
分布正常细胞光镜下区分。这项工作表明，正常光
和荧光显微镜的组合是科学区分
铁皮石斛从经常混淆的物种的快速和有效的技术。