Active workstations such as treadmill desks provide health benefits; however, concerns for the ability to walk safely and work efficiency remain unclear. The purposes of this investigation were to: 1) compare kinematics of treadmill walking (TW) with kinematics of walking while performing computer mousing tasks (WC) using a treadmill desk, and 2) examine work efficiency in terms of the time to complete computer mousing tasks during WC compared to standing (SC). Trunk and lower-extremity kinematic data were obtained from 9 males (23.4 ± 4.2 yrs; 81.7 ± 16.4 kg; 176.3 ± 5.5 cm) and 7 females (23.0 ± 3.3 yrs; 58.4 ± 6.5 kg; 171.7 ± 9.0 cm) using a 10-camera motion capture system. Kinematic data were normalized to the gait cycle and were divided into sub-phases for analysis. Kinematic data of the first and last 10 WC strides were compared to identify shortterm kinematic adaptations (α=0.05). Neither computer task performance (p=0.071) nor walking velocity (p=0.089) was sacrificed during WC compared to SC and TW, respectively. Significant kinematic changes occurred in response to WC (p