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Ethanol fermentation from food wastes containing mainly starch without carrying out sterilization was investigatedby using wild and tolerant yeast, Issatchenkia orientalis MF-121. The MF-121 strain is not a suitable choice for ethanolfermentation from lignocellulosic biomass because it is only capable of fermenting hexoses of glucose, mannose, andfructose to ethanol. Therefore, we first isolated acid- and salt-tolerant yeast that are capable of fermenting variousmonosaccharides to ethanol, and the isolated yeast that showed the ability to ferment ethanol from glucose, mannose,galactose, fructose, and xylose, was identified as Zygoascus hellenicus LK-5G on the basis of the 26S rRNA sequenceanalysis.
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