The mixture was shaken vigorously and allowed to stand for 30 min in the dark, and the absorbance at 517 nm was then measured against a blank with a double beam uv/vis spectrophotometer
The mixture was shaken vigorously and allowed to standfor 30 min in the dark, and the absorbance at 517 nm was thenmeasured against a blank with a double beam uv/vis spectrophotometer