Results (
Persian) 2:
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As shown in Table 1, the primers’ sets used were ST11 and<br>ST15 as a universal gene for Salmonella spp., SI and S4 for S.<br>Enteritidis, and Fli15 and Typ04 for S. Typhimurium according<br>to Soumet et al. (1999). Extraction was carried out using Patho<br>Gene DNA/RNA extraction kit (iNtRON Biotechnology) according to the manufacture’ instructions. Multiplex PCR reaction was done in a total volume of 22 µl containing 11 µl PCR<br>master mix, 1 µl of each primer and 5 µl of the extracted DNA.<br>The thermocycler conditions included initial denaturation at<br>95 oC for 5 min., followed by 35 cycles of 95 oC for 1 min., 48 oC<br>for 1 min., and 72 oC for 1 min., with final extension at 72 oC<br>for 10 min., after amplification, 1% agarose was used for electrophoresis
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