KM and Vmax were determined to be 60.7 mM and 43.5 _M/min for free GODs (FG), 15.2 mM and 4.8 _M/min for immobilized GODs (IG), and 51.2 mM and 5.0 _M/min for IGCB, respectively. After being entrapped into the chitosan beads, the GOD exhibited improved storage and operation stability. The IG and IGCB retained 48% and 64% of their initial activity after 7 reuses, respectively.