Results (
Thai) 1:
[Copy]Copied!
In order to estimate how diverse the mating types inPleurotus eryngii from different regions are, pairingsbetween monokaryons derived from inter- and intragroupswere done. Sixteen and 15 alleles were identified atloci A and B from the 12 strains. In the P. eryngiiKNR2312, widely used for commercial production, fourmating loci, A3, A4, B3, and B4, were determined. Thoseloci, except A3, were found in 4 strains out of 12 strains.To improve breeding efficiency, especially in mating typedetermination, RAPD and BSA were performed to screenfor a mating type specific marker. The SCAR marker 13-22100 was developed based on the RAPD-derived sequencetyping B3 locus. The sequence analysis of 13-22100 revealedthat it contained a conserved domain, the STE3 superfamily,and consensus sequences like the TATA box andGC box. It seems likely that the SCAR marker region is apart of the pheromone receptor gene.
Being translated, please wait..
