Wang et al. (48) fabricated PHBHHx scaffolds by thermally induced phase separation. In this process, polymers are dissolved in 1,4-dioxane under vigorous agitation for 60 min at 656C and the mixture is then kept frozen at –806C for 2 h.
After removal of the solvent via freeze-drying, porous scaffolds are obtained, with a pore size of approximately 60-100 mm in diameter and a porosity of 93±1.4%.
Such porous three-dimensional scaffolds provide additional space for seeded cells to grow.