Method validation 1) Linearity Line

Method validation
1) Linearity
Linearity was determined over the range of 10–50 μg/ml for Salbutamol Sulphate. Accurately measured Stock solution B of Salbutamol Sulphate (1, 2, 3, 4, and 5 ml) was transferred to one set of a series of 10 ml volumetric flasks. To each appropriately labeled flask 0.8 ml of 2N FCR solution and 6 ml of distilled water was added. The volumetric flasks were shaken well for at least 5 min followed by addition of 1.5 ml of 1N NaOH solution. The volume was made up to the mark and stored for 15 min in the dark. A spectrum was recorded by placing drug solutions and diluent in sample and reference cells respectively. The absorbance was measured at 723 nm (Peak maxima) and was plotted vs. concentration to give calibration curve, and regression equation and correlation coefficient was calculated and presented in table 2 and fig. 3. The calibration curve of amplitude of absorbance against concentration of the drug showed linearity.

Results (Indonesian) 2:

Validasi metode
1) Linearitas
Linearitas ditentukan selama rentang 10-50 ug / ml untuk Salbutamol sulfat. Diukur secara akurat solusi Stock B dari Salbutamol sulfat (1, 2, 3, 4, dan 5 ml) dipindahkan ke satu set rangkaian 10 ml labu volumetrik. Untuk masing-masing labu tepat berlabel 0,8 ml larutan 2N FCR dan 6 ml air suling ditambahkan. Termos volumetrik terguncang dengan baik selama sedikitnya 5 menit diikuti dengan penambahan 1,5 ml larutan 1N NaOH. Volume dibuat sampai tanda dan disimpan selama 15 menit dalam gelap. Sebuah spektrum tercatat dengan menempatkan solusi obat dan pengencer dalam sampel dan referensi sel masing-masing. Absorbansi diukur pada 723 nm (Puncak maxima) dan diplot vs konsentrasi untuk memberikan kurva kalibrasi, dan persamaan regresi dan koefisien korelasi dihitung dan disajikan pada tabel 2 dan Ara. 3. Kurva kalibrasi amplitudo absorbansi terhadap konsentrasi obat menunjukkan linearitas.