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Chondrocytes isolated from rabbit articular cartilagebwere inoculated onto numerous PHBHHx/PHB porousscaffolds (54). Total collagen content in all scaffoldsncontaining PHBHHx increased with the duration of incubation. Moreover, the use of PHB/PHBHHx at a ratio of 1:2 in scaffolds resulted in a significant increase in the production of GAG, a major composition of ECM, after 7 days of culture, compared with scaffolds having different component ratios. Therefore, the proportion of PHBHHx in the PHB/PHBHHx composite system is of importance for the promotion of ECM synthesis by articular cartilage 536 H.M. Chang et al.Braz J Med Biol Res 47(7) 2014 www.bjournal.com.brchondrocytes. The inoculated cells grew better on thescaffolds consisting of PHBHHx/PHB at the ratios of 2:1and 1:2 than on those having a 1:1 ratio.
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