deposited onadditionof MeOHwerefilt

deposited onadditionof MeOHwere
fi
ltered off,and the
fi
ltrate
was passed through a Sephadex LH-20 column (1.6×50 cm)
eluted with MeOH (200 ml; fraction of 10 ml) to afford twenty
fractions. The hydrolysates (fractions 10
–
13) were combined
on the basis of TLC behaviors, concentrated under vacuum and
the dried residue was chromatographed on silica gel column
(1.6×40 cm) with gradient CHCl
3
/MeOH (19:1 to 7:3; 1 l;
fraction of 10 ml) to afford the sapogenin
1a
(30 mg) and sugar
mixture (48 mg). The sapogenin
1a
(
Rf
=0.40) and the sugar
mixturewereobtainedfromthefractionsanalyzedbysilicagel-
TLC using the solvent systems (B) CHCl
3
/MeOH (95:5, v/v) and
(C)
n
-BuOH/Me
2
CO/H
2
O (4:5:1, v/v/v), respectively. A sample
of the sugar mixture (1 mg) was dissolved in pyridine (100
μ
l)
and analyzed by silica gel-TLC in the above described solvent
(C). After spraying with orcinol/H
2
SO
4
, glucose and galactose
gave blue spots at
Rf
values of 0.38 and 0.35, respectively.
(3
β
,5
α
,6
β
,25
R
)-Spirostan-3,6-diol (
1a
): Colorless needles
from benzene, mp 239
–
240 °C; [
α
]
D
25
–
72.5° (
c
0.5, CHCl
3
); IR
ν
max
(KBr): 3520, 3250 (OH), 2955, 2930, 2875, 2850 (CH),
1450, 1375, 1340, 1240, 1175, 1075, 1055, 1015, 1005, 980,
955, 915, 900, 860 cm
−
1
(intensity 915
b
900, 25
R
-spiroke-
tal); EIMS:
m
/
z
432 [M]
+
, 139 [C
9
H
15
O]
+
; The
1
H and
13
C
NMR (pyridine-
d
5
) data agreed well with those of literature
data

Results (Indonesian) 1:

deposited onadditionof MeOHwerefiltered off,and thefiltratewas passed through a Sephadex LH-20 column (1.6×50 cm)eluted with MeOH (200 ml; fraction of 10 ml) to afford twentyfractions. The hydrolysates (fractions 10–13) were combinedon the basis of TLC behaviors, concentrated under vacuum andthe dried residue was chromatographed on silica gel column(1.6×40 cm) with gradient CHCl3/MeOH (19:1 to 7:3; 1 l;fraction of 10 ml) to afford the sapogenin1a(30 mg) and sugarmixture (48 mg). The sapogenin1a(Rf=0.40) and the sugarmixturewereobtainedfromthefractionsanalyzedbysilicagel-TLC using the solvent systems (B) CHCl3/MeOH (95:5, v/v) and(C)n-BuOH/Me2CO/H2O (4:5:1, v/v/v), respectively. A sampleof the sugar mixture (1 mg) was dissolved in pyridine (100μl)and analyzed by silica gel-TLC in the above described solvent(C). After spraying with orcinol/H2SO4, glucose and galactosegave blue spots atRfvalues of 0.38 and 0.35, respectively.(3β,5α,6β,25R)-Spirostan-3,6-diol (1a): Colorless needlesfrom benzene, mp 239–240 °C; [α]D25–72.5° (c0.5, CHCl3); IRνmax(KBr): 3520, 3250 (OH), 2955, 2930, 2875, 2850 (CH),1450, 1375, 1340, 1240, 1175, 1075, 1055, 1015, 1005, 980,955, 915, 900, 860 cm−1(intensity 915b900, 25R-spiroke-tal); EIMS:m/z432 [M]+, 139 [C9H15O]+; The1H and13CNMR (pyridine-d5) data setuju juga dengan orang-orang sastradata

Results (Indonesian) 2:

disetorkan onadditionof MeOHwere
fi
disaring off, dan
fi
menyusup
disahkan melalui Sephadex LH-20 kolom (1,6 × 50 cm)
dielusi dengan MeOH (200 ml; fraksi 10 ml) untuk membeli dua puluh
fraksi. Hidrolisat (pecahan 10
-
13) digabungkan
atas dasar perilaku TLC, terkonsentrasi di bawah vakum dan
residu dikeringkan dikromatografi pada kolom silika gel
(1,6 × 40 cm) dengan gradien CHCl
3
/ MeOH (19: 1 sampai 7: 3 ; 1 l;
fraksi 10 ml) untuk membayar sapogenin
1a
(30 mg) dan gula
campuran (48 mg). The sapogenin
1a
(
Rf
= 0,40) dan gula
mixturewereobtainedfromthefractionsanalyzedbysilicagel-
TLC menggunakan sistem pelarut (B) CHCl
3
/ MeOH (95: 5, v / v) dan
(C)
n
-BuOH / Me
2
CO / H
2
O ( 4: 5: 1, v / v / v), masing-masing. Sebuah sampel
dari campuran gula (1 mg) dilarutkan dalam piridin (100
μ
l)
dan dianalisis dengan silika gel-TLC di atas dijelaskan pelarut
(C). Setelah penyemprotan dengan orsinol / H
2
SO
4
, glukosa dan galaktosa
memberi bintik-bintik biru di
Rf
nilai 0,38 dan 0,35, masing-masing.
(3
β
, 5
α
, 6
β
, 25
R
) -Spirostan-3,6-diol (
1a
) : jarum tak berwarna
dari benzena, mp 239
-
240 ° C; [
Α
]
D
25
-
72,5 ° (
c
0,5, CHCl
3
); IR
ν
max
(KBr): 3520, 3250 (OH), 2955, 2930, 2875, 2850 (CH),
1450, 1375, 1340, 1240, 1175, 1075, 1055, 1015, 1005, 980,
955, 915, 900 , 860 cm
-
1
(intensitas 915
b
900, 25
R
-spiroke-
tal); EIMS:
m
/
z
432 [M]
+
, 139 [C
9
H
15
O]
+
; The
1
H dan
13
C
NMR (pyridine-
d
5
) Data setuju dengan baik dengan orang-orang sastra
Data