One kg of dry, mature, whole soybeans was soaked in 3 L
distilled water at 25 8C for 24 h. The soak water was decanted
and the beans washed and ground in 6 L boiling distilled water
in a blender (Waring Division, Dynamics Corporation,
New Hartford, Conn., U.S.A.). The resulting suspension was
filtered through 3 layers of cheesecloth, autoclaved for 15
min at 121 8C and stored at 4 8C until required