How Is SVCv Diagnosed?
Diagnosis of SVCv can be accomplished by several methods.
A direct method is to isolate the virus using fathead minnow (FHM) or epithelioma papillosum of carp (EPC)
cell lines. If the virus is present, it will cause the cells to
degenerate and round (see Figure 2). To confirm that SVCv
is in the cell line, one or more of indirect test methods
should be performed by a federally approved laboratory.
Indirect methods for SVCv diagnosis include enzymelinked
immunosorbent assay (ELISA), virus neutralization,
reverse-transcription polymerase chain reaction (rt-PCR),
and immunofluorescence of suspect tissue.