Results (
Thai) 2:
[Copy]Copied!
the target DNA based on the faster rate of evolution of mtDNA
and because its high copy number (approximately 1,600e6000
molecules per animal cell) compensates for the loss of DNA during
food processing (Zhang et al., 2007). The cytochrome b (cytb) gene
of mtDNA has sufficient interspecific variation in the nucleotide
sequence to allow for differentiation even between closely related
species (Infante et al., 2004). This technique has been widely used
in the identification of vertebrate species, such as mammals (Irwin,
Kocher, & Wilson, 1991), reptiles (Yan et al., 2005), and fish (Chen
et al., 2012; Hsieh et al., 2010). Similarly, Lemer, Aurelle, Vigliola,
Durand, and Borsa (2007) indicated that the cytb fragment was
useful as an identifier of rabbitfish (Siganidae) and named the DNA
barcode of the fish species.
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