A sensitive and specific HPLC metho

A sensitive and specific HPLC method has been developed and validated for the simultaneous determination of
enalapril and hydrochlorothiazide in human plasma using C18 reversed-phase column.The average recoveries
range from 0.005-0.1µg/ml and 0.01-0.2 µg/ml for each drug were 92.7% and 93.3 % respectively. The limit of
detection were 2.5 and 0.14 ng /ml for enalapril and hydrochlorothiazide respectively. the validated method
has been used successfully to study enalapril and hydrochlorothiazide pharmacokinetic, bioavailability and
bioequivalence in 24 adult volunteers.
Keywords: Enalapril Hydrochlorothiazide: HPLC determination; human plasma; bioequivalence;
pharmacokinetics; bioavailability

Results (Indonesian) 1:

Metode KCKT sensitif dan spesifik telah dikembangkan dan divalidasi untuk penentuan simultanenalapril dan hydrochlorothiazide dalam plasma manusia menggunakan kolom C18 terbalik-fase. Pemulihan rata-rataberkisar dari 0.005-0.1µg/ml dan 0,01-0,2 µg/ml untuk masing-masing obat yang 92.7% dan 93,3% masing-masing. BatasDeteksi yang 2,5 dan 0,14 ng /ml untuk enalapril dan hydrochlorothiazide masing-masing. metode divalidasitelah berhasil digunakan untuk belajar ketersediaanhayati pharmacokinetic, enalapril dan hydrochlorothiazide danbioequivalence di 24 orang dewasa relawan.Kata kunci: Enalapril Hydrochlorothiazide: penentuan HPLC; plasma manusia; bioequivalence;farmakokinetik; ketersediaanhayati