layer was removed and the absorbanc

layer was removed and the absorbance was read at 532 nm using spectrophotometer. The MDA concentration in serum sample was determined by adding 0.1 mL diluted serum to 2.5 mL TCA and 1.5 mL TBA at room temperature for 15 min. The MDA concentration in the sample was determined using the MDA standard curve formula:
MDA = MDA concentration from × Vn (4.5 mL)
(nmol/mL) standard curve
VO(0.1 mL) ,
where Vn is the final volume and VO is the early volume.
MDA are then divided by protein (mg/mL) to find the MDA in nmol/mg protein.
LIPID PROFILE ANALYSIS
The lipid profiles observed were the total cholesterol, triglycerides, high-density lipoprotein (HDL) and low-density lipoprotein (LDL). The Commercial kit (SIGMA) was used to run the analysis using Hitachi Chemistry Analyzer at clinical diagnostic laboratory, University Hospital of University Malaya, Kuala Lumpur.
STATISTICAL ANALYSIS
The data collected were in triplicate and presented as average ± standard deviation (SD). Data were statistically evaluated using one-way ANOVA, followed by Dunnett test using STAT software. The values were considered significant when p

Results (Indonesian) 1:

layer was removed and the absorbance was read at 532 nm using spectrophotometer. The MDA concentration in serum sample was determined by adding 0.1 mL diluted serum to 2.5 mL TCA and 1.5 mL TBA at room temperature for 15 min. The MDA concentration in the sample was determined using the MDA standard curve formula:MDA = MDA concentration from × Vn (4.5 mL)(nmol/mL) standard curveVO(0.1 mL) ,where Vn is the final volume and VO is the early volume.MDA are then divided by protein (mg/mL) to find the MDA in nmol/mg protein.LIPID PROFILE ANALYSISThe lipid profiles observed were the total cholesterol, triglycerides, high-density lipoprotein (HDL) and low-density lipoprotein (LDL). The Commercial kit (SIGMA) was used to run the analysis using Hitachi Chemistry Analyzer at clinical diagnostic laboratory, University Hospital of University Malaya, Kuala Lumpur.STATISTICAL ANALYSISThe data collected were in triplicate and presented as average ± standard deviation (SD). Data were statistically evaluated using one-way ANOVA, followed by Dunnett test using STAT software. The values were considered significant when p<0.05.RESULTS AND DISCUSSIONIDENTIFICATION OF PLANT CHEMICAL COMPOUNDSSekarang dari senyawa kimia yang terpisah menggunakan kromatografi lapisan tipis (TLC). Senyawa fenolik yang terdeteksi dengan warna-warna ungu memberikan reagen folin. Komposisi senyawa fenolik yang diidentifikasi dengan Q-TQF MS. tabel 1 menunjukkan bahwa itu berisi senyawa fenolik dari asam quinic, asam galat dan asam caffeic. Kandungan asam Malat di R. tomentosa terungkap dengan massa biaya sebesar 133.0139. Asam galat

Results (Indonesian) 2:

lapisan telah dihapus dan absorbansi dibaca pada 532 nm menggunakan spektrofotometer. Konsentrasi MDA dalam sampel serum ditentukan dengan menambahkan 0,1 mL diencerkan serum 2,5 mL TCA dan 1,5 mL TBA pada suhu kamar selama 15 menit. Konsentrasi MDA dalam sampel ditentukan dengan menggunakan MDA standar rumus kurva:
konsentrasi MDA = MDA dari × Vn (4,5 mL)
(nmol / mL) kurva standar
VO (0,1 ml),
di mana Vn adalah volume akhir dan VO adalah awal volume.
MDA kemudian dibagi dengan protein (mg / mL) untuk menemukan MDA di nmol / mg protein.
lIPID ANALISIS pROFIL
profil lipid yang diamati adalah total kolesterol, trigliserida, high-density lipoprotein (HDL) dan low-density lipoprotein ( LDL). The Commercial kit (SIGMA) digunakan untuk menjalankan analisis menggunakan Hitachi Kimia Analyzer di laboratorium diagnostik klinis, Rumah Sakit Universitas Universitas Malaya, Kuala Lumpur.
ANALISIS STATISTIK
Data yang dikumpulkan adalah dalam rangkap tiga dan disajikan sebagai rata-rata ± standar deviasi (SD). Data statistik dievaluasi menggunakan ANOVA satu arah, dilanjutkan dengan uji Dunnett menggunakan software STAT. Nilai dianggap signifikan ketika p <0,05.
HASIL DAN PEMBAHASAN
IDENTIFIKASI TANAMAN KIMIA SENYAWA
yang hadir senyawa kimia dipisahkan menggunakan kromatografi lapis tipis (TLC). Senyawa fenolik yang terdeteksi dengan Folin reagen memberikan warna ungu. Komposisi senyawa fenolik diidentifikasi dengan Q-TQF MS. Tabel 1 menunjukkan bahwa itu berisi senyawa fenolik asam quinic, asam galat dan asam caffeic. Kandungan asam malat di R. tomentosa itu dijelaskan dengan biaya massa 133,0139. Asam gallic adalah