This study showed that EOs can be incorporated in a polysaccharide matrix without affecting their antimicrobial properties. The coating E + AM showed the best in vitro antimicrobial properties without affecting the sensorial properties of cauliflowers. Moreover, physicochemical analyses performed showed changes in the respiration rates but no major color modifications. Finally, treatments of cauliflowers with coating E + AM allowed a complete inhibition of Listeria after a one-week storage. Therefore, this user-friendly coating could be applied in the food industry as a way to fulfill federal regulation requirements.