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1. Introduction
The Commission of the European Communities disseminated
regulation EC No 178/2002 to indicate food safety as a key policy
priority (Cammà, Di Domenico, & Monaco, 2012). Recently, for
different reasons, including religion, race, allergies, the abuse of
endangered species and the heedless use of cheap or toxic fish,
previously developed techniques in species identification have
been improved in manufactured products (Calvo, Zaragoza, & Osta,
2001; Comi, Iacumin, Rantsiou, Cantoni, & Cocolin, 2005; Yan et al.,
2005). The traditional methods of identifying species of whole fresh
fish were based on their morphological and osteological features.
However, these characteristics are partially or completely removed
during processing (Callejas & Ochando, 2000; Hsieh et al., 2010). At
the same time, thermal treatments, such as cooking and sterilization,
used during processing could cause difficulties in species
identification (Dooley, Sage, Brown, & Garrett, 2005). Currently,
identification procedures generally include determining speciesspecific
protein profiles by electrophoretic techniques, such as
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