When it comes to Western blotting, choosing the right membrane for your application usually spells the difference between success and failure. However, this is usually easier said than done since you need to consider the properties of your protein and the downstream detection steps required in your application before you can determine which membrane can give you the results that you need.
You need to choose a membrane that will deliver signal while resisting background and nonspecific binding. So, how can you do this? You should start by trying to learn a little bit more about the membranes that you use in Western blotting.