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In this paper, the preparation and characterization of some novel nanostructured lipidcarriers for drug delivery are reported. They are obtained by mixing two solid lipids, cetylpalmitate and glyceryl stearate, with three types of vegetable oils: grape seed oil, St. John’swort oil (Hypericum perforatum oil) and sea buckthorn oil. In order to increase theirantioxidant and antimicrobial properties, they are co-loaded with green tea extract byusing a modified high shear homogenization technique. Size distribution and poly-dispersity index of the developed nanostructured lipid carriers determined by the dynamiclight scattering, and corroborated with the results obtained by the transmission electronmicroscopy analysis, confirmed that the structures obtained are at nanoscales. Thecrystallinity behavior of the prepared nanostructured lipid carriers has been studied bydifferential scanning calorimetry; zeta potential measurements show that all loadednanostructures present excellent physical stability. Their antioxidant and antimicrobialproperties evaluated by an appropriate in vitro analysis using the chemiluminescencemethod, and the diffusion disc method, respectively, show that green tea extract could beutilized as a valuable natural source of antioxidant and antimicrobial agent. These newnano-formulations proved to have significant potential for nutritional and pharmaceuticalapplications.
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