A. niger HFD5A-1 isolated from a rotten-decayed Malaysiapomelo showed a significant ability to produce pectinase in sub-merged fermentation culture [17]. A. niger is the most commonlyused fungus for industrial production of pectinolytic enzymesespecially pectinases since it is generally recognized as a safemicroorganism (GRAS). In this study, an efficient system for pecti-nase production has developed by using whole fungal mycelium
immobilized on nylon scouring pad cubes. The nylon fiber shaftsas a carrier provided novel living space for the fungal mycelium togrow and produce pectinase. Thus, the results obtained from thetechnique and it performance in free and immobilized cell can beuseful information for the future scale-up processes for pectinaseproduction.