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Patients and MethodsTwenty-four unrelated Swedish women with advanced epithelial ovarian cancer (mean age 57 y, range 37–85), who had already been treated for first and second line therapy, and 26 unrelated Swedish men with hormone-refractory prostate cancer (mean age 76.5 y, range 64–86) were studied for the genotypes at HLA-A, -B, -C, and -DRB1 loci, in order to choose A2-positive individuals. In the ovarian cancer patients HLA typing was performed on peripheral blood monocytes with a PCR/sequence-specific oligonucleotide hybridization procedure (Olerup SSP AB, Stockholm, Sweden). In the prostate cancer patients HLA-A2 antigen presence was detected with a flow-cytometry-based assay with a monoclonal antibody against HLA-A2, derived from the hybridoma HB-54 (ATCC., Manassas, VA, USA). The percentage of the relative frequencies of HLA alleles in the European countries has been given by each registry through the coordinating center of the Bone Marrow Donors Worldwide Registries in Leiden, The Netherlands. The latitude degrees of the capital city of each country were obtained from Worldatlas.com web site (available at URL http://www.graphicmaps.com/aatlas/world.htm). Age-standardized mortality rates were derived from the Cancer Incidence, Mortality and Prevalence Worldwide web site (available at the URL http://www.dep.iarc.fr/globocan/globocan.html). The frequency of HLA-A2 in the Swedish healthy
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