Results (
Thai) 2:
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Freshly prepared solutions (containing 0.5% of Tween-20) were
foliar sprayed with a mechanical mist sprayer and repeated at
3 dates of the growth cycle: T1 (at pit hardening, 63 days after
full blossom, DAFB), T2 (initial colour changes, 77 DAFB) and T3
(onset of ripening, 98 DAFB). These dates corresponded to key
events in the fruit developmental process, according to previous
experiments (Díaz-Mula et al., 2008). Before T1 treatment 20 fruit
were labelled around the equatorial perimeter of each tree, in
which fruit growth was followed by measuring polar, suture and
cheek diameters, and then fruit volume was calculated as previously
reported (Díaz-Mula et al., 2008). On a weekly basis, from
T1 treatment to commercial harvesting (June 12th and 18th for RR
and BS, respectively), 20 fruit similar in size and colour to those
labelled for each tree (or replicate) were picked for further analytical
determinations: fruit weight, firmness, colour, total soluble
solids (TSS), total acidity (TA). Commercial harvest was established
at 119 and 112 DAFB for BS and RR, respectively, based on fruit
size, colour and the content of total soluble solids characteristic
of each cultivar and with the advice of the field technician. Since
the maximum fruit size was obtained with the concentrations of
MeJA at 0.5 and 2.0 mM for BS and RR trees, respectively, plums
from these treatments were chosen for determining the content of
bioactive compounds (total phenolics and total carotenoids) and
total antioxidant activity and compared with control fruit.
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