The microflora grown on specific media was identified by staining techniques.The predominant microorganism identified was yeast, Saccharomycese cerevisiae.It increases gradually due to its tolerance power towards low pH ,high acidity and ethanol content.Biochemical analysis includes pH,total acidity,reducing sugar,ethanol content etc.In the investigation of biochemical aspects,it was concluded that the pH of sample was decreased whereas the titrable acidity of sample was increased during fermentation process. Wine was prepared after 21 days of fermentation.