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Rev. Sci. Tech. Off. Int. Epiz., 2015, 34 (3), ... - ...No. 19082015-00057-ENEmergency surveillance for novel influenzaA(H7N9) virus in domestic poultry, feralpigeons and other wild birds in BhutanThis paper (No. 19082015-00057-EN) has been peer-reviewed, accepted, edited, andcorrected by authors. It has not yet been formatted for printing and the figures have not beenedited. It will be published in December 2015 in issue 34 (3) of the Scientific and TechnicalReview.T. Tenzin (1)*, S. Tenzin (1), D. Tshering (1), K. Lhamo (1), P.B. Rai (1),N. Dahal (2) & K. Dukpa (1)(1) National Centre for Animal Health, Department of Livestock,Serbithang, Post Box 155, Thimphu, Bhutan(2) Animal Health Division, Department of Livestock, Post Box 113,Thimphu, Bhutan*Corresponding author: tenzinvp@gmail.com;tenzin.tenzin@yahoo.co.ukSummaryFollowing the March 2013 outbreak of novel avian influenzaA(H7N9) virus in humans and the subsequent isolation of the virusfrom chickens, ducks and pigeons in China, concerns were raised thatthe H7N9 virus would spread beyond China through the poultry valuechain linking to a number of bordering countries. For this reason, arapid emergency surveillance exercise took place in Bhutan betweenMay and July 2013 with the objective of determining whetherinfluenza A(H7N9) virus was silently circulating in domestic poultryand wild birds in Bhutan. A total of 1,716 oropharyngeal, tracheal andcloacal swabs together with faecal droppings were collected frompoultry, wild birds and feral pigeons throughout the country; thesesamples included 150 that had been previously collected forsurveillance of influenza A(H5N1) virus. Overall, 733 of the sampleswere tested. A QIAamp(R) Viral RNA Mini kit was used to extractviral RNA from a mix of oropharyngeal, tracheal and cloacal swabs
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