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Phenolic compounds are commonly found in both edible and inedible plants and they have been noticed to have wider biological effects, including antioxidant activity. It has been reported that compounds such as phenolics, flavonoids, which contain hydroxils, are responsible for the radical scavenging activity of most plant. The result of DPPH scavenging activity of ethanolic extracts [Table 2] indicates they are fairly significant scavenger of free radical when compared with standard ascorbic acid measured at IC value. IC value is a parameter able to inhibit 50% of the DPPH. IC value of extracts was compared to the IC of the standard obtained by the same procedure. In comparative analysis, G. sylvestre is the most active and significant (P < 0.05) scavenger than other two, whereas A. bilimbi extract exhibited the lowestactivity among three samples of different species [Table 2]. Extracts show a gradual increase in activity with increase of concentration. DPPH is a commonly used substrate for rapid assessment of antioxidant activity because of its stability and simplicity of the assay. DPPH scavenging capacity of antioxidants is due to hydrogen donating ability. DPPH is stable nitrogen centered free radical, which produces violet color in ethanol solution and accepts an electron or hydrogen radical to become more stable diamagnetic molecule. When a DPPH solution is mixed with a hydrogen atom donor, a stable non-radical form of DPPH is found with simultaneous change in color from violet to pale yellow. This assay gives reliable information about the antioxidant activity of the tested compounds. It is possible to correlate the reduction in the number of DPPH molecules with the number hydroxyl groups.
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