Physical and antimicrobial properties of chitosan films were affected to different extents after incorporation with different amounts of LAE, EDTA and CO. With the addition of CO, the water solubility of films significantly decreased, which suggested the increased water-resistance of films. WVP of control chitosan film was significantly lower than that of films with additional antimicrobials. The tensile strength of films significantly decreased after addition of the antimicrobials, while no significant differences were observed in elongation% among most films. Strong antimicrobial activities of the films incorporated with antimicrobials were detected against both Gram-positive and Gram-negative bacteria after 24 and 48 h in the agar diffusion assay, while it was less significant in the liquid medium. The enhanced antimicrobial activity was observed when incorporating EDTA in films prepared with 0.1 g/100 g LAE. However, addition of CO in the film-forming mixtures resulted in a smaller amount of LAE released from films to inhibit bacteria. Therefore, EDTA can be incorporated in antimicrobial films with LAE to improve the activity inhibiting foodborne pathogens.