Twenty-five grams of each homogeniz

Twenty-five grams of each homogenized sample were placed into separate disposable plastic containers and frozen. The samples were transported from the OSU-SFL to AM Test Laboratories in Redmond, WA, for total mercury analysis. Two-gram aliquots of each sample were digested with 2 ml 16 M HNO3, 4 ml 18 M H2SO4, 1.5 g KMnO4, and 8 ml 5% K2S2O8. Samples sat overnight in reagents and were then cooked in a 98 C water bath for 2 h. After digestion, 2 ml hydroxylamine hydrochloride and 2 ml stannous chloride were added, and the mercury content was measured using a Perkin–Elmer Atomic Absorption Spectrophotometer according to the cold-vapor atomic absorption
EPA method 7471 A, with a detection limit of 0.0001 ppm. Quality control methods included running 1 duplicate and 1 matrix spike per 10 samples analyzed, as well as 1 blank and standard reference material per 20 samples analyzed. No more than a 20% difference was permitted between duplicate samples, and matrix spikes and standard reference spikes required a recovery of 80% or more.

Results (Indonesian) 2:

Dua puluh lima gram masing-masing sampel homogen ditempatkan ke dalam wadah plastik sekali pakai yang terpisah dan beku. Sampel diangkut dari OSU-SFL ke AM Uji Laboratorium di Redmond, WA, untuk analisis merkuri keseluruhan. Aliquots dua gram setiap sampel yang dicerna dengan 2 ml 16 M HNO3, 4 ml 18 M H2SO4, 1,5 g KMnO4, dan 8 ml 5% K2S2O8. Sampel duduk semalam di reagen dan kemudian dimasak dalam 98? C mandi air selama 2 jam. Setelah pencernaan, 2 ml hidroksilamin hidroklorida dan 2 ml stannous klorida ditambahkan, dan konten merkuri diukur menggunakan spektrofotometer serapan Perkin-Elmer Atom menurut serapan atom dingin uap
metode EPA 7471 A, dengan batas deteksi 0,0001 ppm. Metode kontrol kualitas termasuk berjalan 1 duplikat dan 1 matriks lonjakan per 10 sampel yang dianalisis, serta 1 kosong dan standar bahan referensi per 20 sampel yang dianalisis. Tidak lebih dari perbedaan 20% diijinkan antara sampel duplikat, dan matriks paku dan paku acuan standar yang diperlukan pemulihan dari 80% atau lebih.