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Carefully open the QIAamp Spin Column and add 500 μl Buffer AW2 withoutwetting the rim. Close the cap and centrifuge at full speed (20,000 x g; 14,000 rpm)for 3 min. Continue directly with step 8, or to eliminate any chance of possible BufferAW2 carryover, perform step 7a, and then continue with step 8.Note: Residual Buffer AW2 in the eluate may cause problems in downstreamapplications. Some centrifuge rotors may vibrate upon deceleration, resulting in theflow-through, which contains Buffer AW2, coming into contact with the QIAamp SpinColumn. Removing the QIAamp Spin Column and collection tube from the rotor mayalso cause flow-through to come into contact with the QIAamp Spin Column. In thesecases, the optional step 7a should be performed.
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