PHYTOCHEMICAL SCREENING OF THE PLAN

PHYTOCHEMICAL SCREENING OF THE PLANT
PARTS
• TEST FOR ALKALOIDS: Exactly 0.2g of each plant extract was
put into three different test tubes, one sample to one test tube and 5ml of
2% Hydrochloric acid (HCl) was added to each test tube and boiled in a
water bath for 10minutes. The mixture from each test tube was filtered
and 1ml portion each of the filtrate was treated with the following
reagents: (i) Dragendroff’s reagent, (ii) Meyer’s reagent (iii) Wagner’s
reagent (iv) 1% Picric acid. On addition of each reagent to the filtrate, a
red precipitate was formed, which indicates the presence of alkaloids.
• TEST FOR TANNINS: One gramme each of the dried powdered
plant part samples was boiled in 5ml of 45% ethanol in an individual test
tube for 5minutes and then filtered while still hot. The filtrate was
allowed to cool and then treated with the following solutions:

Results (Indonesian) 2:

Penapisan fitokimia TANAMAN
PARTS
• TES UNTUK alkaloid: Tepat 0.2g setiap ekstrak tanaman itu
dimasukkan ke dalam tiga tabung yang berbeda, satu sampel untuk satu tabung reaksi dan 5 ml dari
2% asam klorida (HCl) ditambahkan ke masing-masing tabung reaksi dan rebus dalam
air mandi untuk 10minutes. Campuran dari masing-masing tabung reaksi disaring
dan bagian 1ml masing-masing filtrat diperlakukan dengan berikut
reagen: (i) reagen Dragendroff ini, (ii) pereaksi Meyer (iii) Wagner
reagen (iv) 1% asam Picric. Pada penambahan masing-masing reagen untuk filtrat, sebuah
endapan merah dibentuk, yang menunjukkan adanya alkaloid.
• TES UNTUK tanin: Satu gram masing-masing bubuk kering
tanaman bagian sampel direbus dalam 5 ml dari 45% ethanol di tes individu
tube untuk 5minutes kemudian disaring saat masih panas. Filtrat
dibiarkan dingin dan kemudian diobati dengan solusi berikut: