3.5. Gene regulation by RA signaling in cultured SSCs after RA
induction
According to bioinformatic analysis, four genes (Stra8, Tfrc,
Camk1, and Pdcd4) related to the RA signal and one gene (Scp3)
related to meiosis were chosen for further exploration. SSC
lines were treated with 1 μM of ATRA, and control SSC lines
were treated with the same concentration of dimethyl
sulfoxide (DMSO). As shown in Fig. 4, the expression levels of