water; positive controls were given

water; positive controls were given glibenclamide (5
mg /kg) and insulin (Actrapid HM-40 IU/mL) for type
2 and type 1 model rats respectively. Blood samples
from rats were drawn by amputation of the tail tip.
Blood samples were collected at 0, 60, 120 min for
fasting conditions, at 0, 30, 75 min for simultaneous
feeding of extract with glucose and at 0, 60 and 105
min when the extract was fed 30 min before glucose
load (2.5 g/kg body weight).
Effects of M. indica on intestinal glucose absorption: An
intestinal perfusion technique (Swintosky and
Pogonowska-Wala, 1982) was used to study the effects of
M. indica extracts on intestinal absorption of glucose in
nondiabetic and type 2 diabetic rats fasted for 36 hours
and anesthetized with sodium pentobarbital (50 mg/
kg). The plant extracts were added to a kreb’s solution
(g/L 1.02 CaCl2, 7.37 NaCl, 0.20 KCl, 0.065
NaH2PO4.6H2O, 0.6 NaHCO3, pH 7.4), supplemented
with glucose (54.0 g/L) and perfused at a perfusion
rate of 0.5 mL/min for 30 min through the duodenum.
The perfusate was collected from a catheter set at 40
cm. M. indica extracts were added to Kreb’s solution to
a final conc. of 25 mg/mL so that the amount of extract
in the perfused intestine is equivalent to the dose of
1.25 g/kg. The control group was perfused only with
Kreb’s buffer supplemented with glucose. The results
were expressed as percentage of absorbed glucose,
calculated from the amount of glucose in solution
before and after the perfusion.

Results (Indonesian) 1:

water; positive controls were given glibenclamide (5mg /kg) and insulin (Actrapid HM-40 IU/mL) for type2 and type 1 model rats respectively. Blood samplesfrom rats were drawn by amputation of the tail tip.Blood samples were collected at 0, 60, 120 min forfasting conditions, at 0, 30, 75 min for simultaneousfeeding of extract with glucose and at 0, 60 and 105min when the extract was fed 30 min before glucoseload (2.5 g/kg body weight).Effects of M. indica on intestinal glucose absorption: Anintestinal perfusion technique (Swintosky andPogonowska-Wala, 1982) was used to study the effects ofM. indica extracts on intestinal absorption of glucose innondiabetic and type 2 diabetic rats fasted for 36 hoursand anesthetized with sodium pentobarbital (50 mg/kg). The plant extracts were added to a kreb’s solution(g/L 1.02 CaCl2, 7.37 NaCl, 0.20 KCl, 0.065NaH2PO4.6H2O, 0.6 NaHCO3, pH 7.4), supplementedwith glucose (54.0 g/L) and perfused at a perfusionrate of 0.5 mL/min for 30 min through the duodenum.The perfusate was collected from a catheter set at 40cm. M. indica extracts were added to Kreb’s solution toa final conc. of 25 mg/mL so that the amount of extractin the perfused intestine is equivalent to the dose of1.25 g/kg. The control group was perfused only withKreb’s buffer supplemented with glucose. The resultswere expressed as percentage of absorbed glucose,calculated from the amount of glucose in solutionsebelum dan sesudah perfusi.

Results (Indonesian) 2:

air; kontrol positif diberi glibenklamid (5
mg / kg) dan insulin (actrapid HM-40 ​​IU / mL) untuk jenis
2 dan tipe 1 model tikus masing-masing. Sampel darah
dari tikus yang ditarik oleh amputasi ujung ekor.
Sampel darah diambil pada 0, 60, 120 menit untuk
kondisi puasa, pada 0, 30, 75 menit untuk simultan
makan ekstrak dengan glukosa dan pada 0, 60 dan 105
menit ketika ekstrak itu diberi makan 30 menit sebelum glukosa
beban (2,5 g / kg berat badan).
pengaruh M. indica pada penyerapan glukosa usus: Sebuah
teknik perfusi usus (Swintosky dan
Pogonowska-Wala, 1982) digunakan untuk mempelajari efek
M . ekstrak indica pada usus penyerapan glukosa di
nondiabetes dan tipe 2 tikus diabetes berpuasa selama 36 jam
dan dibius dengan natrium pentobarbital (50 mg /
kg). Ekstrak tanaman ditambahkan ke solusi Kreb
(g / L 1,02 CaCl2, 7.37 NaCl, 0,20 KCl, 0,065
NaH2PO4.6H2O, 0,6 NaHCO3, pH 7,4), dilengkapi
dengan glukosa (54,0 g / L) dan perfusi pada perfusi
tingkat 0,5 mL / menit selama 30 menit melalui duodenum.
perfusate itu dikumpulkan dari kateter ditetapkan pada 40
cm. M. ekstrak indica yang ditambahkan ke solusi Kreb untuk
sebuah conc akhir. dari 25 mg / mL sehingga jumlah ekstrak
di usus perfusi setara dengan dosis
1,25 g / kg. Kelompok kontrol perfusi hanya dengan
penyangga Kreb dilengkapi dengan glukosa. Hasil
dinyatakan sebagai persentase dari glukosa diserap,
dihitung dari jumlah glukosa dalam larutan
sebelum dan sesudah perfusi.