In fact, current response increased with 1,2-benzoquinone concentration
and gelatin layer permeability. The latter was dependent
on the concentration of the gelatin solution, and on the thickness
of the hydrogel film, determined by the volume of the deposited
gelatin solution. Lesser the gelatin solution concentration and
the volume of the deposited drop, higher the layer permeability.
Taking into account these effects, further experiments were performed
in the following optimal experimental conditions: gelatin
solution concentration 6% and volume of 8 L of the gelatin solution
deposited onto the electrode surface, allowing reaching a
maximal current response for an analyte concentration of 5 mM