A validated HPTLC method was develo

A validated HPTLC method was developed for the simultaneous
determination of three angiotensin converting enzyme inhibitors namely
enalapril maleate, moexipril HCl, and ramipril HCl, in binary mixture with
hydrochlorothiazide. Separation was achieved on silica gel 60 F254 HPTLC plates
using mobile phases: as chloroform- ethylacetate- methanol (10:1:5 v/v/v) for
enalapril maleate : hydrochlorothiazide (Rf: 0.27: 0.67); ethylacetate-chloroformglacial
acetic acid (8:2:0.2 v/v/v) for moexipril HCl: hydrochlorothiazide (Rf : 0.15
: 0.45); and benzene- methanol- glacial acetic acid (8:2.5:0.4 v/v/v) for ramipril
HCl : hydrochlorothiazide (Rf: 0.50: 0.65). Measurements were recorded with
UV densitometry at 223, 216 and 210 nm for the simultaneous determination
of the three binary mixtures of enalapril maleate, moexipril HCl, and ramipril
HCl each with hydrochlorothiazide, respectively. The proposed method was
validated according to ICH and was found to be specific, accurate, precise
and robust. It was also successfully applied to the simultaneous determination
of EN; RAM each with HCT in tablet dosage form without interference from
common excipients.
Keywords: Angiotensin converting enzyme; Hydrochlorothiazide; HPTLC;
Binary mixtures

Results (Indonesian) 1:

Metode HPTLC divalidasi dikembangkan untuk simultanpenentuan tiga angiotensin mengubah inhibitor enzim yaituenalapril maleate, moexipril HCl dan ramipril HCl, dalam biner campuran denganhydrochlorothiazide. Pemisahan dicapai di silika gel 60 F254 HPTLC piringmenggunakan mobile fase: sebagai kloroform-ethylacetate-metanol (10:1:5 v/v/v) untukenalapril maleate: hydrochlorothiazide (Rf: 0.27: 0,67); ethylacetate-chloroformglacialasam asetat (8:2:0.2 v/v/v) untuk moexipril HCl: hydrochlorothiazide (Rf: 0.15: 0.45); dan benzena - metanol - glacial asam asetat (8:2.5:0.4 v/v/v) untuk ramiprilHCl: hydrochlorothiazide (Rf: 0,50: 0,65). Pengukuran direkam denganUV Densitometri di 223, 216 dan 210 nm untuk penentuan simultantiga campuran biner enalapril maleate, moexipril HCl dan ramiprilHCl yang masing-masing dengan hydrochlorothiazide, masing-masing. Metoda yang diusulkan adalahdivalidasi sesuai ICH dan ditemukan untuk menjadi spesifik, akurat dan tepatdan kuat. Ia juga berhasil diterapkan untuk penentuan simultanen; RAM masing-masing dengan HCT dalam bentuk dosis tablet tanpa gangguan dariUmum excipients.Kata kunci: Angiotensin converting enzim; Hydrochlorothiazide; HPTLC;Campuran biner